Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. It has not previously been reported. Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause of mycetoma in a diabetic patient when culture and. Proc Natl Acad Sci U S A Sep 29; Draft Genome Sequence of Actinomadura madurae LIID-AJ, Isolated from a Human Mycetoma Case. Vera-Cabrera L.

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The case exemplifies several capabilities of deep sequencing as a clinical diagnostic tool, specifically, deconvoluting the identity of individual organisms within polymicrobial samples, classifying organisms directly from patient specimens without the need for culture, and characterizing nonviable or unculturable organisms in this case, organisms killed by FFPE processing prior to pathology examination.

Wain J, Mavrogiorgou E.

In other projects Wikimedia Commons Wikispecies. Int J Syst Evol Microbiol. Many sites of the human body are colonized by complex communities of microbes adtinomadura both health and various disease states 6.

Consistent with culture results, reads classified as S. Barbeec, d Christopher Rosenthala Brad T.

Nonmycetomic Actinomadura madurae infection in a patient with AIDS.

These chains are of various lengths and can be straight, hooked or spiral. Author information Copyright and License information Disclaimer.

It contains aerobic, Gram-positive, non-acid-fast, non-motile, chemo-organotrophic actinomycetes that produce well-developed, non-fragmenting vegetative mycelia and aerial hyphae that differentiate into surface-ornamented spore chains.

National Center for Biotechnology InformationU. Actinomycetoma in the United States. The patient agreed to this procedure, and was discharged on a course of oral sulfamethoxazole-trimethoprim. From Wikipedia, the free encyclopedia.


Address correspondence to Stephen J. Chronic infections 7 and diabetic foot ulcers 589in particular, can contain highly diverse bacterial populations.

Examination with next-generation sequencing technology of the bacterial microbiota in bronchoalveolar lavage samples after traumatic injury.

Mycetomap 80— In Rippon J, editor. Deep sequencing has previously been used to characterize cultured bacterial isolates 27 and to explore the composition of microbial populations in metagenomic research 28 Footnotes Published ahead of print 9 October Nevertheless, deep sequencing recovered reads 2.

Here, we utilized deep sequencing of 16S rRNA to implicate Actinomadura madurae as the cause actimomadura mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms. This finding is consistent with the results of both culture and Sanger sequencing for the specimen, which were dominated by S.

A punch biopsy specimen of the lesion was submitted to pathology, revealing marked acute and chronic inflammation and an inclusion of abundant actknomadura structures consistent with aerobic actinomycetes Fig. Approximately 4 years prior to the current presentation, the diagnosis of actinomycotic mycetoma was adtinomadura established by histology, and she was initiated on antibiotic therapy with penicillin mg orally [p.

PCR products were purified using 0. Support Center Support Center. A modified two-step treatment for actinomycetoma. Actinobacteria Bacteria genera Actinobacteria stubs.

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The patient was referred to the Madufae Disease clinic at Harborview Medical Center for further evaluation. A number of other organisms were also detected, reflecting the polymicrobial nature of such specimens 5. A portion of the specimen was concurrently submitted for culture in order to establish the identity of the aerobic actinomycetes-like nadurae and to evaluate its antibiotic sensitivities; however, the culture quickly became overgrown by Staphylococcus aureuspreventing culture for slower-growing aerobic actinomycetes species.


Nucleotide sequence accession number. Actinomadura madurae is an aerobic actinomycete which is best known worldwide as the cause of actinomycotic mycetomas. Monsaasa Mimosa Chaua Lindley A. Neoscytalidium dimidiatum actinomadurs Aspergillus flavus. The patient noted some retreat of the lesions and improvement in her pain and pruritis while on this treatment, and she was transitioned to oral penicillin mg p. Identification of aerobic actinomycetes of clinical importance.

Next-generation actinomsdura in clinical microbiology. Actinomycetoma in the United States. Retrieved from ” https: Mycetoma in South India: In this case report, we have utilized the approach to investigate the causative agent of biopsy-proven actinomycotic mycetoma.

Actinomadura madurae – Global Catalogue of Microorganisms

Please review our privacy policy. Madudae, on imaging, the patient’s osteomyelitis was found to involve all bones of the foot, including the calcaneus. Comparison of species distribution and antimicrobial susceptibility of aerobic actinomycetes from clinical specimens.

J Lab Clin Med. Intravenous penicillin 20 million units i. This Actinobacteria -related article is a stub. Treatment of actinomycetoma foot—our experience with ten patients. Polymicrobial specimens may be difficult or even impossible to fully characterize by techniques in common clinical use: Sequence reads originating from A.